Thermo Fisher Scientific GCN5 Monoclonal Antibody (2GC-2C11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

ChIP assay (ChIP)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2GC-2C11

Immunogen

Synthetic peptide coupled to ovalbumin corresponding to amino acids 118-132 of human GCN5: CKCNGWKNPKPPTAP. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCN5,uniProtId:Q92830-1,ncbiNodeId:9606,antigenRange:118-132,antigenLength:837,antigenImageFileName:MA3-046_GCN5_Q92830-1_House_mouse.svg,antigenImageFileNamePDP:MA3-046_GCN5_Q92830-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2606246

Product Specific Information

MA3-046 detects GCN5 in human samples.

MA3-046 has been succesfully used in western blot and ChIP applications.

Immunogen is a synthetic peptide corresponding to residues 118-132 of human GCN5

Target Information

SAGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histoneacetylase complex which has Gcn5p as catalyst subunit and functions overlapping with the fundamental transcription factor TFIID which has Taf1p as catalyst subunit. However, SAGA and TFIID have different allotment and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also, SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds), TBP related protein group (4 kinds), TAF protein group (5 kinds that also pertain to TFIID), and other protein groups (>6 kinds). As its molecular function, there is the chemical modification of histone, or the recruitment by direct interaction of transcription regulating factor on DNA, or the control of transcription starting reaction by TBP. Gcn5p that show histoneacetylase activity is one kind of the above-mentioned Ada protein group, and in the case of budding yeast it is composed of 439 amino acid residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.