Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

Description:
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR via immobilized UCHT1 induces intracellular signaling leading to activation and proliferation.

Applications Reported:
For use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (100 µL final volume). Cell count may vary from 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is optimized for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to reduce non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light due to photo-induced oxidation sensitivity.

Fixation Compatibility:
Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency.

Fluorescence Properties:

• Excitation: 350 nm
• Emission: 564 nm
• Laser: Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits encoded on chromosome 11. These subunits contain cytoplasmic tails associated with signal transduction molecules. CD3 is found on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but absent in B and NK cells. CD3 gene defects are linked to immunodeficiency disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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