Thermo Fisher Scientific CD25 Monoclonal Antibody (CD25-4E3), eFluor 450, eBioscience
인간 및 비인간 영장류 CD25 인식 단일클론 항체로, eFluor 450 형광체 결합 제품입니다. Flow Cytometry에 최적화되어 있으며, 활성화된 T세포 및 조절 T세포 표지에 사용됩니다. 405 nm 레이저에 적합하며, 4°C 암소 보관 권장.
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Thermo Fisher Scientific CD25 Monoclonal Antibody (CD25-4E3), eFluor 450, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 9 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD25-4E3 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574011 |
Available Formats
- APC
- APC-eFluor 780
- Brilliant Violet 605
- FITC
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
This CD25-4E3 monoclonal antibody reacts with human CD25, also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including regulatory T cells), B cells, and macrophages. It associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high-affinity IL-2 receptor, which mediates IL-2 signaling linked to lymphocyte proliferation and survival.
CD25 serves as a marker for Hodgkin’s disease and has been associated with autoimmune diseases such as Type I diabetes.
This antibody is cross-reactive with rhesus monkey.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test (in 100 µL final volume). Suitable for 10⁵–10⁸ cells/test.
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure the instrument supports Violet laser detection.
Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD25 (IL2 receptor alpha chain, IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. It forms a high-affinity receptor complex with IL2RB and IL2RG. IL2RA expression is monoallelic in mature thymocytes and plays a crucial role in immune regulation. IL2 stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Mutations in IL2RA are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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