Thermo Fisher Scientific CD25 Monoclonal Antibody (eBio3C7 (3C7)), eFluor 450, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (eBio3C7 (3C7)), eFluor 450

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1 µg/test
Publications: View 6 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• Functional Grade
• Request custom conjugation

Product Specific Information

The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Ra).
CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 only with low affinity, but associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high-affinity IL-2 receptor.
Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody.
CD25 plays a role in lymphocyte differentiation and activation/proliferation.

The epitopes recognized by eBio3C7, PC61.5, and eBio7D4 are all different, allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤1 µg/test (100 µL final volume).
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®.
Emits at 445 nm and is excited with the Violet laser (405 nm).
Ensure instrument compatibility.

Optical Properties:

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation.
IL2RA forms a high-affinity receptor complex with IL2RB and IL2RG.
Homodimeric IL2RA chains result in low-affinity receptors, while IL2RB homodimers form medium-affinity receptors.
IL2 expression in mature thymocytes is monoallelic, ensuring precise gene regulation.
IL2 is primarily produced by mature T cells and acts as a growth and differentiation factor, regulating cell death.
It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation.
Disruption of similar genes in mice leads to ulcerative colitis-like disease, indicating a role in immune response regulation.
Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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