Thermo Fisher Scientific CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), eFluor 450, eBioscience
인간 CD24 항원을 인식하는 eBioSN3 (SN3 A5-2H10) 단클론 항체로, eFluor 450으로 결합되어 유세포분석(Flow Cytometry)에 최적화됨. 405/445 nm 파장에서 형광 발현, 4°C에서 암소 보관. 연구용으로만 사용.
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Applications
Immunocytochemistry (ICC/IF)
- Tested dilution: Not specified
- View 2 publications
Flow Cytometry (Flow)
- Tested dilution: 5 µL (0.25 µg)/test
- View 23 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSN3 (SN3 A5-2H10) |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219685 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 805
- FITC
- NovaFluor Blue 510 / 555 / 585 / 690 / 760
- NovaFluor Red 725
- NovaFluor Yellow 570 / 590 / 660 / 755
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645
- Request custom conjugation available
Product Specific Information
The eBioSN3 (SN3 A5-2H10) monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HAS).
This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells. P-selectin (CD62P) has been reported to bind to CD24.
Applications Reported:
- Flow cytometric analysis
Applications Tested:
- Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Fluorochrome Information:
- eFluor® 450 is an alternative to Pacific Blue®
- Emission: 445 nm
- Excitation: 405 nm (Violet laser)
- Ensure instrument compatibility
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from bone marrow pro-B cells through mature surface Ig-positive B cells.
Plasma cell expression is low or negative. CD24 is also found on most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas.
It may play a role in regulating B-cell proliferation, maturation, and autoimmunity control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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