Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), eFluor 450, eBioscience
마우스 CD24 항원을 인식하는 M1/69 클론의 Rat IgG2b 단일클론 항체로, eFluor 450 형광체 결합. Flow Cytometry에 최적화되어 있으며, 405/445 nm에서 Violet 레이저로 검출 가능. 면역세포 분석 및 B세포 발달 연구에 적합.
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Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 52 publications |
| T-Cell Activation (TCA) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/69 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1311174 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 711 / 786
- FITC
- Functional Grade
- NovaFluor Blue 510 / 610-30S
- NovaFluor Red 685 / 710
- NovaFluor Yellow 570 / 590 / 660
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645
- Custom conjugation available upon request
Product Specific Information
The M1/69 monoclonal antibody reacts with mouse CD24 (Heat Stable Antigen, HSA), a 35–50 kDa molecule anchored to the plasma membrane via phosphatidylinositol. CD24 is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells.
This glycosylated molecule shows variable molecular mass depending on cell lineage. CD24 expression is used to resolve stages of B lymphopoiesis in mouse bone marrow and has been reported to bind to P-selectin (CD62P).
Applications Reported: Flow cytometric analysis
Applications Tested: Verified in mouse splenocytes; optimal performance ≤ 0.25 µg/test (100 µL final volume). Cell number range 10⁵–10⁸ cells/test; titration recommended.
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm (Violet laser). Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD24 is a GPI-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells to mature B cells. Plasma cells show low or no expression. CD24 is also found in B-lineage acute lymphoblastic leukemias and B-cell lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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