Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), eFluor 450, eBioscience
인간 CD20 단백질을 인식하는 2H7 클론의 마우스 모노클로날 항체로, eFluor 450 형광 표지. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 445 nm에서 방출. B세포 활성화 연구용으로 적합.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), eFluor 450, eBioscience
Applications
Immunohistochemistry (IHC)
- Tested Dilution: Not specified
- View 4 publications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
- View 19 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1633385 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711
- eFluor 506
- FITC
- NovaFluor Blue 610-70S / 660-120S
- NovaFluor Red 710 / 725
- NovaFluor Yellow 590 / 660
- PE, PE-Cyanine5 / 5.5 / 7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed on developing and mature B cells, but not on plasma cells. CD20 may play a role in B-cell activation and is occasionally found at low levels on a subset of mature T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage is 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure the instrument supports this fluorochrome.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, as well as a subset of T lymphocytes and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts and colocalizes with BCR during activation. It remains on the cell surface after antibody ligation, making it a valuable target for therapeutic B-cell depletion. CD20 dysfunction is linked to Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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