Thermo Fisher Scientific Collagen Type V Polyclonal Antibody, Biotin

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at 4°C prior to opening.
• Stable at 4°C as an undiluted liquid; dilute only prior to immediate use.
• For extended storage, mix with an equal volume of glycerol, aliquot, and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes, which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin-embedded tissues.
This antibody reacts with most mammalian Type V collagens and shows negligible cross-reactivity with Type I, II, III, IV, and VI collagens. Non-specific cross-reaction with other human serum or extracellular matrix proteins is negligible.

Target Information

Type I collagen is the fibrillar collagen found in most connective tissues and is a major component of the extracellular matrix. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, and idiopathic osteoporosis.
Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are linked to dermatofibrosarcoma protuberans, a skin tumor resulting from unregulated expression of the growth factor.
Two transcripts, resulting from alternate polyadenylation signals, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.