Thermo Fisher Scientific Collagen Type III Polyclonal Antibody, Biotin

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.
Some class-specific anti-collagens may be specific for three-dimensional epitopes, which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin-embedded tissues.
This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V, and VI collagens.
Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Target Information

Collagen III is a fibrillar collagen found in extensible connective tissues such as skin, lung, and the vascular system, often in association with Type I Collagen.
Mutations in this gene are associated with Ehlers-Danlos syndrome type IV and with aortic and arterial aneurysms.
Although alternate transcripts have been detected, they are the result of mutations that alter splicing, often leading to exclusion of multiple exons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.