Thermo Fisher Scientific Laminin Polyclonal Antibody, Biotin
Biotin으로 결합된 Laminin 폴리클로날 항체로, 인간 Laminin에 특이적입니다. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합하며, 높은 순도와 낮은 교차 반응성을 제공합니다. 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Thermo Fisher Scientific Laminin Polyclonal Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Non-reduced 400 kDa human laminin. Immunization occurred after purity assessment of native protein in non-reducing SDS-PAGE system. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with 1.0 mL of deionized water (or equivalent).
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I–VI Collagens or Fibronectin.
Non-specific cross-reaction with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
Target Information
Laminin antibodies are widely used to label blood vessels and basement membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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