Thermo Fisher Scientific Phospho-DARPP-32 (Ser102) Polyclonal Antibody
DARPP-32 단백질의 Ser102 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(P)에서 검증됨. 인간, 마우스, 랫트 반응성. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PPP1R1B (Accession Q9UD71), corresponding to amino acid residues around phosphorylated Ser102. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816062 |
Product Specific Information
This antibody detects endogenous levels of DARPP-32 only when phosphorylated at Ser102, a site historically referenced as Ser97.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regulated ~32 kDa phosphoprotein associated with dopaminoceptive neurons. When phosphorylated on Thr34, it inhibits Protein Phosphatase I; when phosphorylated on Thr75, it acts as a PKA inhibitor. Phosphorylation of DARPP-32 plays a critical role in dopaminergic neurotransmission and is implicated in the actions of alcohol, caffeine, and certain antidepressants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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