Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE-eFluor 610, eBioscience
인간 및 사이노몰거스 원숭이 CD154(CD40 Ligand)을 검출하는 단클론 항체로, PE-eFluor 610 형광 표지. Flow cytometry에 최적화되어 있으며, 488–561 nm 레이저에 적합. 고순도 친화 크로마토그래피 정제, 4°C 암소 보관.
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Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE-eFluor 610, eBioscience
Applications
- Flow Cytometry (Flow): 5 µL (0.5 µg)/test
- Tested by flow cytometric analysis of stimulated normal human peripheral blood cells
- Defined test volume: 100 µL (cell number 10⁵–10⁸ cells/test, empirically determined)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Cynomolgus monkey, Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 24-31 |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574583 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Request custom conjugation
Product Specific Information
Description:
The 24-31 monoclonal antibody reacts with human CD154 (CD40 Ligand, gp39), a 39 kDa transmembrane glycoprotein of the TNF superfamily expressed transiently by activated T cells. CD154 interacts with CD40 on antigen-presenting cells (B cells, monocytes/macrophages, dendritic cells) to mediate T-dependent B-cell activation and cycle entry. Clone 24-31 cross-reacts with cynomolgus monkey CD154.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells. Recommended use: 5 µL (0.5 µg)/test.
Fluorophore Information:
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red® equivalent). Ensure instrument compatibility.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
488–561 nm / 607 nm / Blue, Green, Yellow-Green Laser.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD40 Ligand (CD40-L, CD154):
A membrane glycoprotein expressed on activated T cells. Stimulates B cell proliferation and immunoglobulin secretion, induces cytokine production and tumoricidal activity in monocytes, and co-stimulates activated T cell proliferation with IFN-γ, TNF-α, and IL-2 production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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