Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), PE-eFluor 610, eBioscience
인간 및 붉은털원숭이의 CD152(CTLA-4)를 인식하는 단클론 항체로, PE-eFluor 610으로 결합되어 유세포분석용으로 최적화됨. 활성화된 T세포의 CTLA-4 발현 분석에 적합하며, 광민감성이 있어 암실 보관 필요. 연구용 전용 제품.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (1.0 µg)/test
- Publications: View 9 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14D3 |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802382 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The 14D3 monoclonal antibody reacts with human CD152 (CTLA-4), a protein structurally related to CD28. CTLA-4 is expressed on activated T cells and binds CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28. While CD28 acts as a costimulatory molecule, CTLA-4 inhibits T-cell activation by reducing IL-2 secretion and proliferation. The cytoplasmic domain of CTLA-4 contains ER retention motifs, leading to intracellular localization upon TCR signaling.
This antibody also recognizes rhesus monkey CTLA-4 and exhibits inhibitory activity.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and validated for flow cytometry using stimulated human peripheral blood cells
- Recommended Use: 5 µL (1.0 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)
Fluorochrome Information:
PE-eFluor 610 is excited by 488–561 nm lasers and emits at 607 nm. Recommended detection filter: 610/20 band pass (PE-Texas Red equivalent). Ensure instrument compatibility.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal loss of brightness and FRET efficiency.
Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor primarily expressed on CD4⁺ T cells. It binds CD80 and CD86 with higher affinity than CD28 and delivers inhibitory signals to T cells, limiting immune activation. Intracellular CTLA-4 is also found in regulatory T cells and contributes to their suppressive function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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