Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (16B1), PE-eFluor 610, eBioscience
인간 CD144(VE-cadherin)을 인식하는 16B1 클론 단일클론 항체로, PE-eFluor 610 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, HUVEC 세포에 검증되었습니다. 488–561 nm 레이저로 여기, 607 nm에서 방출되며 광 민감성을 주의해야 합니다.
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Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (16B1), PE-eFluor 610, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | - |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16B1 |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848492 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- PE
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The 16B1 antibody reacts with human CD144 (VE-cadherin, cadherin-5), a calcium-dependent adhesion molecule localized at endothelial cell junctions. It plays roles in vascular permeability and remodeling.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted, tested on Human Umbilical Vein Endothelial Cells (HUVEC)
- Use at 5 µL (0.25 µg) per test (100 µL final volume)
- Applicable for 10⁵–10⁸ cells/test
Fluorochrome Information:
PE-eFluor 610 is excited by 488–561 nm lasers and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red equivalent). Ensure instrument compatibility.
Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal brightness loss.
Excitation/Emission: 488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Target Information
VE-cadherin is a calcium-dependent cell-cell adhesion glycoprotein located on chromosome 16. It consists of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It contributes to endothelial cell junction organization and cohesion. An alternative splice variant has been reported.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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