Thermo Fisher Scientific BID Monoclonal Antibody (OTI3E6), TrueMAB
인간 BID 단백질을 인식하는 단일클론 항체로 Western blot에 최적화됨. HEK293T 세포에서 생산된 재조합 단백질을 면역원으로 사용. 무보존제, 동결건조 형태로 제공되며 -20°C에서 보관. 연구용으로 세포사멸 관련 단백질 분석에 적합.
- 판매단위
- pk
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Applications
- Western Blot (WB)
Tested Dilution: 1:400–1:4,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E6 |
| Immunogen | Full length human recombinant protein of human BID produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
BID (BH3-interacting domain death agonist) is a pro-apoptotic member of the BCL-2 family. It heterodimerizes with BAX or BCL2 and mediates mitochondrial damage induced by caspase-8 (CASP8).
Upon activation by CASP8 cleavage, the C-terminal fragment (p15) translocates to mitochondria, triggering cytochrome c release and apoptosis.
BID acts as a molecular link between caspase-8 activation and mitochondrial death machinery.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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