Thermo Fisher Scientific BID Monoclonal Antibody (OTI3E9), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. It is a member of the BCL-2 family of cell death regulators and mediates mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria to trigger cytochrome c release, leading to apoptosis.
In living cells, BID exists in an inactive cytosolic form and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once cleaved, the C-terminal 15 kDa fragment (p15) translocates to mitochondria, triggering cytochrome c release and cell death. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.