Thermo Fisher Scientific H3K14ac Recombinant Rabbit Monoclonal Antibody (RM130)
Histone H3K14ac 인산화 부위를 특이적으로 인식하는 recombinant rabbit monoclonal antibody입니다. Western blot, ICC, ChIP 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공합니다. Protein A 정제 및 glycerol 함유 PBS buffer에 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 2–10 µg/mL |
| Luminex (LUM) | 0.1–0.5 µg/mL |
| Peptide Array (Array) | 0.25 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM130 |
| Immunogen | Acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys14) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661913 |
Product Specific Information
This antibody reacts to the Histone H3 K36M mutant and shows no cross-reactivity with wild-type Histone H3.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Higher specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure that organizes DNA. Histones are essential for transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes various post-translational modifications, such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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