Thermo Fisher Scientific ANAPC11 Monoclonal Antibody (OTI1B11), TrueMAB
인간 ANAPC11 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot, IHC, ICC 등 다양한 응용에 적합. 동결건조 형태로 제공되며, 재구성 및 결합 실험에 용이. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B11 |
| Immunogen | Full length human recombinant protein of human ANAPC11 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Together with the cullin protein ANAPC2, ANAPC11 forms the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase controlling progression through mitosis and the G1 phase.
The APC/C complex mediates ubiquitination and degradation of target proteins, primarily forming Lys-11-linked polyubiquitin chains and, to a lesser extent, Lys-48- and Lys-63-linked chains.
May recruit E2 ubiquitin-conjugating enzymes to the complex. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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