Thermo Fisher Scientific Phospho-ULK1 (Ser450) Polyclonal Antibody
ULK1 단백질의 Ser450 인산화 상태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot, IHC, ICC/IF에 사용 가능. 인간 및 마우스 반응성. PBS/glycerol buffer에 보존되며 -20°C에서 저장. 연구용으로만 사용.
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- PA5105421
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ULK1 (Accession O75385), corresponding to amino acid residues around phosphorylated Ser450 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816849 |
Product Specific Information
Antibody detects endogenous levels of ULK1 only when phosphorylated at Ser450.
Target Information
ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital cellular response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls its regulatory function and localization. Knockdown of ULK1 inhibits autophagic response and rapamycin-induced autophagy, consistent with its role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and for ULK1 stability. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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