Thermo Fisher Scientific Phospho-IRE1 alpha (Tyr628) Polyclonal Antibody
Phospho-IRE1 alpha (Tyr628) 항체로 인간, 마우스, 랫트 시료에서 인산화된 IRE1 검출에 적합. Western blot, IHC, ICC/IF 적용 가능. 고순도 정제, 1 mg/mL 농도, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ERN1 (Accession O75460), corresponding to amino acid residues around phosphorylated Tyr628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816853 |
Product Specific Information
This antibody detects endogenous levels of IRE1 only when phosphorylated at phospho Y628.
Target Information
ERN1 (serine/threonine kinase) or endoplasmic reticulum to nucleus signaling 1 is a human homologue of the yeast Ire1 gene product. It possesses intrinsic kinase and endoribonuclease activities, playing a key role in ER-based stress signaling. ERN1 controls IRE1 proteolysis in mammalian cells and is essential for fetal viability in extraembryonic cells. IRE1 acts as a sensor of unfolded proteins in the ER, initiating the unfolded protein response by splicing XBP1 mRNA into a potent transcriptional activator. While null mutants are viable, IRE1 is essential under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p has no effect under normal conditions but induces apoptosis under ER stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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