Thermo Fisher Scientific Carbonic Anhydrase IX Monoclonal Antibody (CA9, 781)
CA9 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, Western blot 및 IHC(Paraffin)에서 1–2 µg/mL로 사용 가능. 인간 및 말 시료 반응성. Recombinant human CA9로 면역화된 항체이며, hypoxia 관련 연구 및 CA IX 발현 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Horse, Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CA9, 781 |
| Immunogen | Recombinant human CA9 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is one of the most inducible genes under hypoxia via HIF-1 alpha, due to its stability and membrane localization. Carbonic anhydrases regulate pH in normal tissues by controlling hydrogen ion (H⁺) flux.
Blockade of CA IX increases cell death under hypoxia, making CA IX a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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