
Thermo Fisher Scientific Proximal Nephrogenic Antigen (Carbonic Anhydrase IX) Monoclonal Antibody (PN-15)
Proximal Nephrogenic Antigen (Carbonic Anhydrase IX) 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. Human 및 Horse 반응성, Protein A/G 정제, 4°C 보관, 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Horse, Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PN-15 |
| Immunogen | Microsomal fraction of human renal cortical tissue homogenate (Carbonic Anhydrase IX, UniProt ID: Q16790-1, amino acids 1–459) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in mammalian tissues and plays a key role in pH regulation.
CA IX is a hypoxia-inducible membrane-associated isoform regulated by HIF-1α. It serves as a reliable histochemical marker of hypoxia, as its inhibition enhances cell death under hypoxic conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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