Thermo Fisher Scientific DLL4 Polyclonal Antibody
DLL4 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot, IHC, ELISA에 사용 가능. 인간 DLL4에 반응하며, 혈관 발달 연구 및 종양 혈관 관련 연구에 적합. 고순도 친화 크로마토그래피 정제 제품으로 안정적인 저장 및 재현성 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution / Concentration |
|---|---|
| Western Blot (WB) | 1:300–1:1500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL |
| ELISA | 1:3000–1:15,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the internal region of human DLL4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2092965 |
Product Specific Information
- Predicted to react with chimpanzee, mouse, and rat based on sequence homology.
- Suggested positive control for IHC: human ovary.
- In Western blot, detects a ~74 kDa band in pancreatic cell lysate.
- Rabbit anti-Human Delta-like Protein 4 antibody detects human DLL4 (Delta-like ligand 4), also known as Delta-4.
Target Information
Delta-like ligand 4 (DLL4), also known as Delta-4, is a homolog of the Drosophila delta protein and functions as a transmembrane ligand for the Notch1 receptor. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF) and acts as a negative feedback regulator to control angiogenic sprouting and promote vascular network differentiation.
DLL4 is strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer. Inhibition of DLL4 increases vascular proliferation but results in defective maturation, leading to decreased tumor growth without apparent toxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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