Thermo Fisher Scientific c-Fos Monoclonal Antibody (5B10), FITC
FITC로 결합된 c-Fos 단일클론 항체로 세포 내 단백질 발현 분석에 적합. Western blot 및 IHC에 사용 가능. 인간 c-Fos N말단 펩타이드 기반 면역원으로 제작. 4°C 암조건 보관, 형광 특성(Ex/Em: 498/517 nm).
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Thermo Fisher Scientific c-Fos Monoclonal Antibody (5B10), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B10 |
| Immunogen | Synthetic peptide from the N-terminal of Human c-Fos (aa. 280–380) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45887 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects a 62 kDa band. Observed molecular weight of the band is higher than the predicted 41 kDa band due to post-translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog (c-Fos) belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of genes involved in cellular signal transduction, proliferation, and differentiation.
Phosphorylation of c-Fos occurs at multiple sites in response to stimuli such as growth factors and insulin. The ERK MAPK kinase phosphorylates c-Fos at Threonine 232, regulating its nuclear localization and transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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