Thermo Fisher Scientific c-Fos Monoclonal Antibody (4A3), FITC
FITC로 표지된 c-Fos 단클론 항체로, Western blot과 IHC에 적합. 인간 c-Fos N-말단 합성 펩타이드로부터 유래된 항원 사용. 498/517 nm 형광 특성. 4°C 암조건 보관, 연구용 한정.
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Thermo Fisher Scientific c-Fos Monoclonal Antibody (4A3), FITC
Applications and Tested Dilution
- Western Blot (WB): 1:1,000
- Immunohistochemistry (IHC): 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG2 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A3 |
| Immunogen | Synthetic peptide from the N-terminal of Human c-Fos (aa. 280–380) |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45884 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects a 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post-translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the fos transcription factor family. It is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of genes involved in signal transduction, proliferation, and differentiation.
Phosphorylation of c-Fos occurs at multiple sites in response to stimuli such as growth factors and insulin. The ERK MAPK kinase phosphorylates Threonine 232, regulating nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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