Thermo Fisher Scientific KDEL Monoclonal Antibody (2D6), PE
KDEL 서열을 인식하는 마우스 단일클론 항체로, PE 형광 표지되어 세포 내 단백질의 ER 위치 확인에 적합합니다. Western blot에서 1:1,000 희석으로 검증되었으며, Human, Mouse, Rat 시료에 반응합니다. 단백질 G로 정제된 고품질 항체입니다.
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Applications and Tested Dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D6 |
| Immunogen | KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932330 |
Additional Formats
- Unconjugated (MA5-27581)
- APC (MA5-45873)
- FITC (MA5-45874)
- PerCP (MA5-45875)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45876 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects KDEL tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL), or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
GRP78 (78 kDa) and GRP94 (94 kDa), as well as protein disulfide isomerase (PDI), share the C-terminal KDEL sequence.
This sequence is necessary for ER retention and helps reduce secretion of proteins from the ER, mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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