Thermo Fisher Scientific c-Fos Monoclonal Antibody (5B10), APC
APC 형광 표지된 c-Fos 단클론 항체로, Western blot과 IHC에 적합합니다. 인간 c-Fos N-말단 펩타이드(aa 280-380)를 면역원으로 제작되었으며, 62 kDa 밴드를 검출합니다. 세포 신호전달 및 전사 인자 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunohistochemistry (IHC)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B10 |
| Immunogen | Synthetic peptide from the N-terminal of Human c-Fos (aa 280–380) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45886 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects a 62 kDa band. Observed molecular weight of the band is higher than the predicted 41 kDa band due to post-translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog (Cellular proto-oncogene Fos, c-Fos) belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of genes involved in cellular signal transduction, proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli including growth factors and insulin at multiple sites. The best-known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK, which regulates nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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