Thermo Fisher Scientific c-Fos Monoclonal Antibody (4A3), PerCP
c-Fos 단백질을 검출하기 위한 PerCP 결합 단클론 항체. Western blot과 IHC에 적합하며, 인간 c-Fos N-말단 펩타이드(aa 280-380)를 면역원으로 제작됨. 4°C에서 보관, 1 mg/mL 농도의 액상 제품. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A3 |
| Immunogen | Synthetic peptide from the N-terminal of Human c-Fos (aa 280–380) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
A 1:1,000 dilution of MA5-45885 was sufficient for detection of c-Fos in 10 µg of serum-starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects a 62 kDa band. The observed molecular weight of the band is higher than the predicted 41 kDa due to post-translational modifications.
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of several genes involved in cellular signal transduction, proliferation, and differentiation.
c-Fos is phosphorylated by various stimuli, including growth factors and insulin, at multiple sites. ERK MAPK is a known upstream kinase for phosphorylation at Threonine 232, which regulates nuclear localization and transcriptional activity of c-Fos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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