Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (MAR-1), Biotin, eBioscience
FcεRIα 단백질을 인식하는 MAR-1 단클론 항체로, 비오틴 표지되어 Flow Cytometry에 최적화됨. 마우스 반응성, IgE 매개 알레르기 반응 연구에 활용 가능. 0.5 mg/mL 농도의 액상 형태로 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 publications |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 25 publications |
| Immunoprecipitation (IP) | - | 1 publication |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAR-1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466783 |
Additional Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available on request
Product Specific Information
Description:
The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit (FcεRIα), an IgE-binding subunit that lacks signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. It forms a tetrameric complex with one beta and two gamma subunits, which contain ITAM motifs essential for signal transduction. The FcεRI complex triggers IgE-mediated allergic reactions.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of the MC/9 mouse mast cell line. Recommended use ≤ 0.125 µg per test (100 µL final volume). Optimal antibody concentration should be titrated for each experiment.
Filtered through a 0.2 µm membrane post-manufacturing.
Target Information
FceR1 (FcεRIα):
A subunit of the high-affinity IgE receptor, forming a tetrameric complex (αβγ₂). The receptor mediates allergic responses by coupling allergen-bound IgE with mast cells, leading to histamine and mediator release responsible for allergy symptoms such as those in hay fever and asthma.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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