Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 450
• eFluor 660
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac, which is recognized by CD22.

Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of Con A-stimulated mouse splenocytes at ≤0.5 µg per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GL7 is a cell-surface protein expressed on activated T and B lymphocytes, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines Ramos and Daudi. Expression varies by strain (BALB/c > C57BL/6). GL7 serves as a marker for mouse germinal center B cells. The GL7 epitope is a sialic acid glycan moiety (Neu5Ac) recognized by CD22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.