Thermo Fisher Scientific MHC Class I (H-2Kk) Monoclonal Antibody (AF3-12.1.3), Biotin, eBioscience
마우스 H-2Kk MHC Class I 항원을 인식하는 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. Biotin으로 표지되어 있으며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용되며, 4°C 암소 보관을 권장합니다.
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Thermo Fisher Scientific MHC Class I (H-2Kk) Monoclonal Antibody (AF3-12.1.3), Biotin, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF3-12.1.3 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1944402 |
Product Specific Information
This AF3-12.1.3 monoclonal antibody reacts with the H-2Kk MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g., b, f, j, k, p, q, r, s, u, and v) has not been observed.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.125 µg per test (100 µL final volume). Cell number may range from 10^5 to 10^8 cells/test. Antibody should be titrated for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein is an MHC class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are essential in Immunopeptidomics, aiding in neoantigen identification and characterization via high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
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