Thermo Fisher Scientific MHC Class I (H-2Kk) Monoclonal Antibody (AF3-12.1.3), Biotin, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Kk) Monoclonal Antibody (AF3-12.1.3), Biotin, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test

Product Specifications

Product Specific Information

This AF3-12.1.3 monoclonal antibody reacts with the H-2Kk MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g., b, f, j, k, p, q, r, s, u, and v) has not been observed.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.125 µg per test (100 µL final volume). Cell number may range from 10^5 to 10^8 cells/test. Antibody should be titrated for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are essential in Immunopeptidomics, aiding in neoantigen identification and characterization via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Additional Formats

• eFluor 450
• Request custom conjugation