Thermo Fisher Scientific CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), Biotin, eBioscience
마우스 CD137 Ligand(4-1BBL)을 인식하는 TKS-1 단클론 항체로, Biotin으로 표지되어 있습니다. Flow Cytometry 분석에 적합하며, 항원제시세포 활성화 연구에 활용됩니다. 0.5 mg/mL 농도의 액상 제품으로 4°C 암소 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 1 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TKS-1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466788 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
Description:
The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor (TNF) superfamily. 4-1BBL is expressed by activated antigen-presenting cells including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) delivers a costimulatory signal important in T cell–APC interaction.
Applications Reported:
The TKS-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
The TKS-1 antibody has been tested by flow cytometric analysis of resting and 2–3 day (IgM & CD40)-activated mouse splenocytes. Recommended usage is ≤1 µg per test (100 µL final volume). Optimal antibody titration is recommended for best assay performance. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Target Information
CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. It acts as a co-stimulatory molecule expressed on antigen-presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137 (4-1BB), is expressed on various cell types including activated T cells and inflamed endothelial cells. Engagement of CD137 with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on most B-cell lymphomas (mantle cell, follicular, diffuse large B-cell) and some carcinoma cell lines, indicating a role in T cell–tumor cell interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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