Thermo Fisher Scientific ITFG2 Monoclonal Antibody (OTI3A3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

ITFG2 is a component of the KICSTOR complex, functioning in the amino acid-sensing branch of the TORC1 signaling pathway.
It recruits the GATOR1 complex to lysosomal membranes, enabling interaction with GATOR2 and RAG GTPases.
Acts upstream of RAG GTPases to negatively regulate mTORC1 signaling in absence of amino acids.
Loss of KICSTOR leads to constitutive localization and activation of mTORC1 at lysosomes.
The complex may also play a role in glucose-mediated regulation of mTORC1.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.