Thermo Fisher Scientific STAT4 Monoclonal Antibody (OTI2F3), TrueMAB

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

STAT4 was originally identified using degenerate primers complementary to conserved regions of other STAT proteins. STAT4 shares 52% similarity with STAT1 and 47% with STAT3. It can be tyrosine phosphorylated by Jak1 or Jak2, forming homo- or heterodimers with other STAT family members. Phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and additional serine phosphorylation enhances transcriptional activity.

STAT4 expression is mainly found in thymus, spleen, and testis. It is activated by interleukin 12 (IL-12), which is required for T-cell–independent induction of IFN-gamma and Th1 immune response development. STAT4-deficient mice show impaired Th1 and enhanced Th2 differentiation. In response to viral infection, IFN-α/β activation of STAT4 is essential for IFN-γ production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.