Thermo Fisher Scientific IL1RA Polyclonal Antibody, PeproTech
Human IL-1RA 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western Blot과 ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로 면역 및 염증 반응 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-1RA |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929816 |
Product Specific Information
AA Sequence of recombinant protein:
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-1RA. Anti-Human IL-1RA-specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix.
Sandwich ELISA:
To detect Human IL-1RA by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-1RA (500-P209Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-1RA.
Western Blot:
To detect Human IL-1RA by Western Blot, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human IL-1RA is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a cytokine receptor belonging to the interleukin 1 receptor family. It acts as a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor type I (IL1R1/IL1RA). It plays a key role in cytokine-induced immune and inflammatory responses. This gene, along with IL1R2, IL1RL2, and IL1RL1, forms a cytokine receptor gene cluster mapped to chromosome 2q12.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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