Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), APC, eBioscience
마우스 CD3e 인식 단일클론 항체(145-2C11)로 T 세포 표면 TCR 복합체 분석에 사용. APC 형광 표지로 유세포분석에 적합. 0.25 µg/test 권장. 4°C 보관, 동결 금지. 연구용 전용.
- 카탈로그번호
- 17-0031-xx (4개 옵션)
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 6 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 |
| Flow Cytometry (Flow) | 0.25 µg/test | 219 |
| Functional Assay (Functional) | - | 2 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host/Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 145-2C11 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469313 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Biotin
- Brilliant UV 496 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 610-70S / 660-120S
- NovaFluor Red 685 / 700 / 710
- NovaFluor Yellow 610 / 660 / 690 / 700 / 730
- PE, PE-Cyanine5 / 5.5 / 7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 780
- Custom conjugation available
Product Specific Information
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with CD3 gamma and delta, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 145-2C11 to TCR can initiate intracellular signaling leading to activation, proliferation, or apoptosis depending on assay conditions. It is widely used as a phenotypic marker for mouse T cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thymocytes and splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation/Emission: 633–647 nm / 660 nm (Red Laser).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD3 subunit complex is crucial for transducing antigen-recognition signals in T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta chains, encoded by closely linked genes on human chromosome 11. These subunits associate with cytoplasmic signaling molecules via double tyrosine-based motifs. CD3 is expressed on most T lymphocytes and thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.