Thermo Fisher Scientific USP16 Monoclonal Antibody (OTI1B4), TrueMAB

Thermo Fisher Scientific USP16 Monoclonal Antibody (OTI1B4), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution

• 1:200–1:4000

Publications

• [References not provided]

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Modification of target proteins by ubiquitin participates in diverse biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. Ubiquitin attachment can alter subcellular localization or protein activity. Deubiquitination, catalyzed by deubiquitinating enzymes (DUBs), includes four families:

1. Ubiquitin C-terminal hydrolases
2. Ubiquitin-specific processing proteases (USPs)
3. OTU-domain ubiquitin-aldehyde-binding proteins
4. Jab1/Pad1/MPN-domain-containing metallo-enzymes

Among these, USPs are the most prevalent and conserved across evolution. They release ubiquitin from conjugated proteins and possess conserved catalytic domains with Cys and His boxes, while variable N- and C-terminal extensions contribute to substrate recognition and localization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.