Thermo Fisher Scientific IL-6 Polyclonal Antibody, PeproTech
Rat IL-6를 검출하기 위한 Rabbit Polyclonal Antibody로, Western Blot, ELISA, Neutralization 등에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 시약. -20°C에서 보관하며 연구용으로만 사용.
- 카탈로그번호
- 500-P73-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-6 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific IL-6 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| ELISA | 0.5–2.0 µg/mL | View publications |
| Neutralization (Neu) | – | View 4 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat IL-6 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930725 |
Product Specific Information
AA Sequence of Recombinant Protein:
MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIL EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-6. Anti-Rat IL-6-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-6 matrix.
Sandwich ELISA:
To detect Rat IL-6 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. When used with PeproTech Biotinylated Anti-Rat IL-6 (500-P73Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Rat IL-6.
Western Blot:
To detect Rat IL-6 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Rat IL-6 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
IL-6 encodes a cytokine involved in inflammation and B-cell maturation. It acts as an endogenous pyrogen, inducing fever in autoimmune or infectious conditions. Expressed mainly at sites of acute and chronic inflammation, IL-6 triggers a transcriptional inflammatory response via interleukin 6 receptor alpha. Elevated IL-6 levels are associated with diabetes mellitus, systemic juvenile rheumatoid arthritis, Kaposi sarcoma, and viral infections such as COVID-19.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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