Thermo Fisher Scientific C-Jun Monoclonal Antibody (OTI1C8), TrueMAB
인간 c-Jun 단백질을 인식하는 마우스 단클론 항체입니다. Western blot에 최적화되어 있으며, 동결건조 형태로 제공됩니다. PBS 기반 완충액에 8% 트레할로스를 포함하며, 보존제는 포함되지 않습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C8 |
| Immunogen | Recombinant fragment expressed in E. coli corresponding to amino acids 103–204 of human c-Jun |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
c-Jun is a transcription factor that binds the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
When phosphorylated by HIPK3, it enhances NR5A1 activity, increasing steroidogenic gene expression upon cAMP signaling stimulation.
It is also involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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