Thermo Fisher Scientific IL-3 Monoclonal Antibody (OTI3B11), TrueMAB
IL-3 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF에 사용 가능. Human 시료에 반응하며, carrier-free 형태로 제공되어 결합 실험에 적합. 동결건조 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 20–152 of human IL-3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Its functions are often enhanced by co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% sequence identity, and their bioactivity is species-specific — human IL-3 is inactive on mouse cells.
IL-3 supports proliferation, differentiation, and apoptosis of various hematopoietic cells and may also possess neurotrophic activity associated with neurologic disorders.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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