Thermo Fisher Scientific CD16 Recombinant Mouse Monoclonal Antibody (B88-9)
CD16을 인식하는 Mouse IgG1 형 재조합 단일클론 항체로, 인간 NK 세포 표면 수용체 연구에 적합합니다. ICC/IF, Flow Cytometry, IP 등 다양한 분석에 사용 가능하며, HEK293 발현 시스템에서 생산된 고순도 항체입니다.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: Assay-dependent
Flow Cytometry (Flow)
- Tested Dilution: Assay-dependent
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | B88-9 |
| Immunogen | This antibody was raised against FCgRIII expressing cells. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
B88-9 binds to human CD16 at an epitope comprised of LEKDSVTK (aa 21–27), SWKNTALH (aa 113–120), FHHNS (aa 134–138), and VGSKN (aa 158–162).
hCD16 is recognized by B88-9 when both bound and unbound to hIgG1.
B88-9-bound CD16 cannot be bound by hIgG1.
Target Information
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in immune complex clearance and cellular cytotoxicity.
The gene FCGR3A shares high similarity with FCGR3B, located on chromosome 1.
FCGR3A is expressed on NK cells as a transmembrane glycoprotein, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) via a phosphatidylinositol (PI) linkage.
Mutations in FCGR3A are associated with:
- Immunodeficiency 20
- Susceptibility to recurrent viral infections
- Systemic lupus erythematosus
- Alloimmune neonatal neutropenia
Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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