Thermo Fisher Scientific CD56 (NCAM) Monoclonal Antibody (TULY56), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: -

Product Specifications

Additional Formats

• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 800
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 690
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

This TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells (NKT cells).

Staining with TULY56 does not block binding of CMSSB, suggesting the two antibodies recognize different epitopes. TULY56 performs better after fixation and permeabilization than CMSSB. The antibody crossreacts with Rhesus macaque.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with a 355 nm UV laser. Verify instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in dark with minimal impact on brightness and FRET efficiency.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family involved in cell adhesion and neural development. It is expressed in neurons, NK cells, and subsets of T cells, playing roles in cell migration, axonal growth, and synaptic plasticity. Polysialic modification reduces adhesion. CD56 interacts with extracellular matrix components such as laminin and integrins.

It is found on most neuroectodermal-derived cell lines and tumors (retinoblastoma, medulloblastoma, astrocytoma, neuroblastoma) and serves as a sensitive neuroendocrine marker for tumors such as ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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