Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet 661, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages, dendritic cells, and activated T cells. It binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on Raji cells (5 µL/test)
• Cell Range: 10⁵–10⁸ cells/test

Brilliant Ultra Violet™ 661 (BUV661) is a tandem dye emitting at 660 nm, optimized for cytometers with ultraviolet (355 nm) lasers. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness or FRET efficiency.

• Excitation: 350 nm
• Emission: 660 nm
• Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, and monocytes. Dysfunction of CD80 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures.

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