Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), DyLight 550
인간 Nestin 단백질을 인식하는 마우스 단클론 항체로 DyLight 550 형광으로 표지됨. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 인간 시료에 특이하며 설치류 및 토끼와 교차 반응하지 않음. 줄기세포 및 신경세포 연구용으로 이상적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | A fragment of the human nestin protein corresponding to residues 1464–1614 |
| Conjugate | DyLight™ 550 |
| Excitation/Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 1.68 mg/mL |
| Purification | Protein G |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153719 |
Additional Formats Available
- Biotin (MA5-16102)
- DyLight 488 (MA5-16104)
- DyLight 650 (MA5-16103)
- HRP (MA5-16105)
- Custom conjugation available upon request
Product Specific Information
This antibody does not cross-react with rodent or rabbit.
Target Information
Nestin is a Class VI intermediate filament abundantly expressed in the developing central nervous system (CNS) and early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker useful for evaluating neovascularity in tumors.
Highly specific antibodies to human Nestin can aid in:
- Characterizing progenitor cells differentiating into distinct lineages
- Enhancing therapeutic potential of human neural stem/progenitor cells in CNS disease or injury
- Identifying neuroepithelial tumor cells
Nestin expression is also observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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