Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), HRP
인간 Nestin 단백질을 인식하는 HRP 결합 단클론 항체로, Western blot 및 면역조직화학(IHC)에 적합합니다. 인간 시료에 특이적으로 반응하며, 설치류 및 토끼와 교차 반응하지 않습니다. 줄기세포 및 종양 내 신생혈관 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | A fragment of the human nestin protein corresponding to residues 1464–1614 |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 2.23 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_11152644 |
Additional Formats
Product Specific Information
This antibody does not cross-react with rodent or rabbit.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It is widely used as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin serves as a superior angiogenic marker for evaluating neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can assist in characterizing progenitor cells differentiating into distinct lineages, enhancing the therapeutic potential of human neural stem/progenitor cells in CNS disease or injury treatment, and identifying neuroepithelial tumor cells. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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