Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), DyLight 488
인간 Nestin 단백질을 인식하는 마우스 단클론 항체로 DyLight 488 형광으로 표지됨. 신경줄기세포, 종양혈관세포 등에서 Nestin 발현 연구에 적합. 다양한 응용(WB, IHC, ICC, Flow)에서 사용 가능. 단백질 G로 정제된 고순도 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
| Flow Cytometry (Flow) | Assay-Dependent | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | A fragment of the human nestin protein corresponding to residues 1464–1614 |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1.24 mg/mL |
| Purification | Protein G |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152370 |
Additional Formats Available
Product Specific Information
This antibody does not cross-react with rodent or rabbit.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin is also a superior angiogenic marker for evaluating neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in:
- Characterizing progenitor cells differentiating into distinct lineages
- Enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury
- Identifying neuroepithelial tumor cells
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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