Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Biotin, eBioscience
Mouse Fas Ligand(CD178)을 인식하는 MFL3 단클론 항체로, Biotin 결합형입니다. Flow Cytometry에 최적화되어 있으며 0.25 µg/test로 사용됩니다. PBS buffer에 보존되며 4°C 암소 보관이 권장됩니다. Fas/FasL 매개 세포사멸 연구에 적합합니다.
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Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 12 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MFL3 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466794 |
Available Formats
- APC
- APC-eFluor 780
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein belonging to the TNF family. FasL is expressed by activated mouse T cells, and interaction with its receptor CD95 induces Fas-mediated apoptosis. Human FasL is known to be cleaved by matrix metalloproteinases (MMPs) to generate a 26 kDa soluble form, while mouse sensitivity to MMPs remains unreported.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated for flow cytometry using mouse FasL transfected cells and activated T cells at ≤0.25 µg/test (in 100 µL final volume). Optimal antibody titration is recommended.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD178 (Fas ligand, FasL) is a type-II membrane protein with its N-terminus in the cytoplasm and C-terminal region extracellular. Its receptor, FasR, is a type-I membrane protein of the TNF/NGF receptor family. Interaction between FasL and FasR triggers apoptosis via receptor oligomerization and downstream signaling. CD178 exists as both membrane-bound (45 kDa) and soluble (26 kDa) forms, the latter generated through metalloproteinase cleavage.
CD178 is mainly expressed in activated T-lymphocytes and NK cells, as well as immune-privileged tissues (testis, eye). Expression is also found in thymus, liver, ovary, lung, heart, and kidney. Fas/FasL-mediated apoptosis is critical in antiviral immune responses and immune regulation. Dysregulation of CD178 expression is associated with atherosclerosis, inflammatory diseases, AIDS, hepatitis, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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