
Thermo Fisher Scientific Mouse anti-Rat IgM (Heavy chain) Secondary Antibody, FITC
Flow Cytometry용 FITC 결합 Mouse anti-Rat IgM (Heavy chain) 2차 항체로, 높은 특이성과 민감도를 제공. Rat IgM μ heavy chain에 특이적이며 교차 반응 없음. Affinity chromatography로 정제되어 안정적이며, 신호 증폭 및 다양한 검출 시스템에 활용 가능.
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Applications and Tested Dilution
- Flow Cytometry (Flow): 10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | MARM-4 |
| Immunogen | Polyclonal rat IgM |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538304 |
| Target | IgM (Heavy chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
For FACS analysis, use 50 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Rat IgM Heavy Chain antibody, clone MARM-4 recognizes the μ heavy chain of rat immunoglobulin and does not cross-react with other immunoglobulin classes.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility by enabling the use of various detection systems (e.g., HRP, AP, fluorescence) and provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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