Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1 Recombinant Rabbit Monoclonal Antibody (HL134)
SARS-CoV-2 Spike Protein S1을 특이적으로 인식하는 재조합 토끼 단클론 항체. WB, IHC, ICC/IF, ELISA 등 다양한 응용 가능. SARS-CoV 및 MERS-CoV 스파이크 단백질과 교차반응 없음. HEK293 발현, Protein A 정제, 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HL134 |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.00 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890586 |
Product Specific Information
Specificity: This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on internal testing.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses ranging from mild cold-like symptoms to severe respiratory distress and death. The Spike Protein consists of S1 and S2 domains and serves as the main surface antigen of the virus. The glycosylated Spike protein can be detected in infected cell culture supernatants using antisera from SARS patients.
The S1 domain contains a receptor-binding domain (RBD) that binds specifically to ACE2 receptors on target cells. Diversity among coronaviruses is reflected in variations of Spike proteins, influencing receptor interactions and fusion mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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