Thermo Fisher Scientific Macrophages/Monocytes Monoclonal Antibody (ER-HR3)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Flow Cytometry (Flow)

1:50

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2c

Class

Monoclonal

Type

Antibody

Clone

ER-HR3

Immunogen

Murine monocytes and macrophages.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076962

Product Specific Information

MA1-40078 detects Monocytes/Macrophages from mouse samples.

MA1-40078 is specially reactive to the monocytes and macrophages located in hemopoietic organs. Whereas all other leukocytes are ER-HR3 negative, up to 70% of circulating monocytes stain positive with this monoclonal antibody. Furthermore, about 30% of bone marrow cells express the antigen. Other positive macrophages can be found in the splenic red pulp, in the mesenteric lymphoid paracortex, interfollicular areas of Peyer`s patches and bone marrow. Few positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen.

MA1-40078 has been successfully used in immunohistochemistry (paraffin and frozen) and flow cytometry applications.

The MA1-40078 immunogen is mouse monocytes and macrophages.

Target Information

Macrophages are a large phagocytic cell found in stationary form in the tissues or as a mobile white blood cell, especially at sites of infection. Monocytes are a large phagocytic white blood cell with a simple oval nucleus and clear, grayish cytoplasm. Monocytes and macrophages are found in interdigitating cells and activated microglia cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.